Table 1.
0 | 10 μM DPC | 100 μM DPC | |
---|---|---|---|
kcat (s−1) | 0.3 ± 0.2 | 0.22 ± 0.06 | 0.09 ± 0.02 |
KM (μM) | 7 ± 5 | 4 ± 2 | 3 ± 1 |
Ksi (μM) | 5 ± 4 | 12 ± 6 | 60 ± 40 |
R2 | 0.92 | 0.90 | 0.95 |
Kinetic parameters calculated from fitting the observed data to the substrate inhibition equation (KaleidaGraph 4.0; Synergy). Values indicate the average ± standard error from the curve fit. The experiment was independently repeated twice with similar results.