Table 3.
The effect of Lucilia sericata antimicrobial peptides used alone and in combination on the E. coli JM83 cell surface.
| Control | Lser-Stomoxyn (0.25 µM) | Lser-PRP2 (50 µM) | Stomoxyn (0.25 µM) + Lser-PRP2 (50 µM) |
|
|---|---|---|---|---|
| Roughness [nm] | 0.843 (± 0.22) | 1.102 (± 0.373)b*** |
1.002 (± 0.354)a* | 0.856 (± 0.295)ab |
| Young's modulus [MPa] | 2458 (± 24.52) | 2601 (± 32.19)** | 2166.14 (± 480.23) | 2791.5 (± 429.81) |
| Adhesion forces [nN] | 0.174 (± 0.04) | 0.236 (± 0.079)a*** | 0.306 (± 0.160)*** | 0.3541 (± 0.169)a*** |
The bacteria were incubated alone (control) or in the presence of Lser-stomoxyn (0.25 µM) or Lser-PRP2 (50 µM), or both and then analyzed by atomic force microscopy. The results are presented as means of 25 fields from each sample ± standard deviation (SD). The same letters indicate statistically significant differences between the peptide-treated experimental groups (Mann-Whitney U test). The asterisks indicate statistically significant differences of the peptide-treated bacteria compared to controls (*p < 0.05; **p ≤ 0.01; ***p ≤ 0.001).