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. 2020 Apr 24;11:546. doi: 10.3389/fmicb.2020.00546

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Copyright © 2020 Muselius, Sukumaran, Yeung and Geddes-McAlister.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Secretome profiling of K. pneumoniae under iron limitation. (A) Principal component analysis; clustering based on treatments (component 1) and biological reproducibility (component 2). (B) Volcano plot depicting all proteins identified in limited and replete (10 μM iron) conditions, highlighting proteins with significant increases or decreases in abundance during 10 μM iron-replete (blue) vs. iron-limited (red) conditions. Lon protease demonstrated the largest decrease in abundance relative to the replete conditions (>8-fold) (highlighted). (C) Volcano plot depicting all proteins identified in limited and replete (100 μM iron) conditions, highlighting proteins with significant increases or decreases in abundance during 100 μM iron-replete (green) vs. iron-limited (red) conditions. Lon protease is highlighted. (D) Volcano plot depicting all proteins identified in replete (10 and 100 μM iron) conditions, highlighting proteins with significant increases or decreases in abundance during 100 μM (green) vs. 10 μM (blue) iron conditions. (E) Pie chart illustrating cellular compartments and signal peptides (undesignated by cellular compartment, but contain signal peptide) of secreted proteins. (F) Venn diagram of K. pneumoniae proteins identified in this secretome study (orange) vs. proteins identified in previous profiling of outer membrane vesicles (yellow). (G) STRING network analysis of significantly different proteins with an increase in abundance during replete vs. limited conditions. Protein clusters highlighted. (H) STRING network analysis of significantly different proteins with a decrease in abundance during replete vs. limited conditions. Protein clusters highlighted. For volcano plots: Student’s t-test, p-value ≤ 0.05; FDR = 0.05, S0 = 1.