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. Author manuscript; available in PMC: 2020 Aug 12.
Published in final edited form as: Cancer Cell. 2019 Aug 12;36(2):194–209.e9. doi: 10.1016/j.ccell.2019.07.003

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-Actin Santa Cruz sc-147778; RRID: AB_626632
Anti-PRMT5 Abeam Abeam ab109451; RRID: AB_10863428
Anti-SDMA Cell signaling #13222; RRID: AB_2714013
Anti-ADMA Cell signaling #13522; RRID: AB_2665370
Anti-PRMT1 Cell signaling CST #2449; RRID: AB_2237696
Anti-MMA Cell signaling #8015; RRID: AB_10891776
Anti-EZH2 Cell signaling CST #5246; RRID: AB_10694683
Anti- γH2Ax Cell signalling CST #9718; RRID: AB_2118009
Biological Samples
AML primary patient samples Princess Margaret Hospital, Memorial Sloan Kettering Cancer Center, & Weill Cornell College of Medicine N/A
Chemicals, Peptides, and Recombinant Proteins
GSK3203591 SGC Toronto https://thesgc.org/chemical-probes/GSK591
MS023 SGC Toronto https://www.thesgc.org/chemical-probes/MS023
EPZO15666 Tocris Cat. No. 6516
GSK3368712 GSK N/A
E7107 H3 Biomedicine Inc. N/A
I-BRD9 SGC Toronto https://www.thesgc.org/chemical-probes/l-BRD9
SGC-CBP30 SGC Toronto https://www.thesgc.org/chemical-probes/CBP30
BI-9564 SGC Toronto https://www.thesgc.org/chemical-probes/BI-9564
I-CBP112 SGC Toronto https://www.thesgc.org/chemical-probes/l-CBP112
MS049 SGC Toronto https://www.thesgc.org/chemical-probes/MS049
UNC0638 SGC Toronto https://thesgc.org/chemical-probes/UNC0638
SGC707 SGC Toronto https://www.thesgc.org/chemical-probes/SGC707
GSK484 SGC Toronto https://www.thesgc.org/chemical-probes/GSK484
10X2 SGC Toronto https://www.thesgc.org/chemical-probes/IOX2
GSK-J4 SGC Toronto https://www.thesgc.org/chemical-probes/gskj1
Vorinostat Cayman chemicals CAS No_ 149647-78-9
GSK2 334470 Cayman chemicals CAS No_ 1227911-45-6
SGC0946 SGC Toronto https://www.thesgc.org/chemical-probes/SGC0946
PD0332991 Cayman chemicals CAS No_ 827022-32-2
CCT244747 Cayman chemicals CAS No_ 1404095-34-6
PF-477736 Cayman chemicals CAS No_ 952021-60-2
BMS-265246 Cayman chemicals CAS No_ 582315-72-8
Irinotecan Cayman chemicals CAS No_ 100286-90-6
SCH772984 Cayman chemicals CAS No_ 942183-80-4
Alitretinoin Cayman chemicals CAS No_ 5300-03-8
CX-4945 Cayman chemicals CAS No_ 1009820-21-6
Phenoxodiol Sigma CAS No_ 81267-65-4
5Z-7-oxozeaenol Cayman chemicals CAS No_ 253863-19-3
Critical Commercial Assays
Caspase-Glo. 3/7 luminescence assay Promega G8090
Cell titer Glo Promega G7570
MTS assay Promega G3582
PTMScan® Asymmetric Di-Methyl Arginine Motif [adme-R] Kit Cell Signaling Technologies Kit #13474
PTMScan® Symmetric Di-Methyl Arginine Motif [sdme-RG] Kit Cell Signaling Technologies Kit #13563
PTMScan Mono-Methyl Arginine 13 Motif [mme-RG] Kit Cell Signaling Technologies Kit #12235
Deposited Data
RNA-seq data This paper GEO:GSE123774
MS-proteomics data This paper PRIDE: PXD012007
Experimental Models: Cell Lines
Human: K562 cells ATCC CLL-243
Human: THP1 cells ATCC TIB-202
Human: M0LM13 cells DSMZ ACC 554
Human: U937 cells ATCC CRL-1593.2
Human: K562 SRSF2P95H/WT knockin cells Horizon Discovery N/A
Human: K562 SF3B1K700E/WT knockin cells Horizon Discovery N/A
Human: TF1 cells ATCC CRL-2003
Human: 0CI-AML5 cells DSMZ ACC 247
Human: F-36P cells DSMZ ACC 543
Human: GDM-1 cells ATCC CRL-2627
Human: KO52 cells JCRB CVCL-1321
Human: HNT34 cells DSMZ ACC 600
Human: MonoMac6 cells DSMZ ACC 124
Human: 5–16 Cre20 (SRSF2 P95L) (Chang et al., 2018) N/A
Human: N-2.12 (isogenic normal) (Chang et al., 2018) N/A
Experimental Models: Organisms/Strains
Mice: Srsf2P95H/+ (Kim et al., 2015). N/A
Mice: MLL-AF9/Vav-cre Srsf2WT/WT (Kim et al., 2015). N/A
Mice: MLL-AF9/Vav-cre Srsf2P95H/WT (Kim et al., 2015). N/A
Mice: Vav-cre transgenic mice (B6.Cg-Tg(Vav1-icre)A2Kio/J) The Jackson Laboratory JAX: 008610
Oligonucleotides
Human EZH2 F (Lee et al., 2016) TTTCATG CAACACCC AACACT
Human EZH2 R (Lee et al., 2016) CCCTGCTTCCCTATCACTGT
Human ATF2 F This paper AGTTACATGTGAATTCTG CCAG G
Human ATF2 R This paper CTCAAATG G ACTCG CCAACTC
Human INTS3 F This paper ATGCCAAG CTGGCTTTGTTTT
Human INTS3 R This paper TCCGACATATGGTTGTCCATCTC
Human TRPT1 F This paper GG CCAACCAG G GCCATT
Human TRPT1 R This paper ATCACCAG CCAAG GAAAG GG
Human HDAC7 F This paper GG AAG AATCCACTG CTCCGA
Human HDAC7 R This paper GACTGGG CAAAGTGG AAG GG
Human LEF1 F This paper CCACCCATCCCGAGAACATC
Human LEF1 R This paper AGG CTTCACGTG CATTAGGT
Anti-sense oligonucleotide SCR This paper CGGUGUGUGUAUCAUUCUCUAGUGU
Anti-sense oligonucleotide EZH2 This paper U GAAU C U U CU G U CCAAAAU CCAACAGGCAAU AUA
sgRNA 1 sequence for EZH2 This paper TTATCAG AAG G AAATTTCC G
sgRNA 2 sequence for EZH2 This paper TTATG ATG G GAAAGTACACG
Recombinant DNA
LentiCRISPR v2 N/A Addgene: 52961
Software and Algorithms
Cytoscape 3.4.0 (Shannon et al., 2003) https://cytoscape.org/
Reactome Fl (Wuetal., 2014) N/A
plogo web application (O’Shea et al., 2013) N/A
hmLINKER (Massignani et al., 2019) N/A
hmSEEKER (Massignani et al., 2019) N/A
Prism 7.0 Graphpad https://graphpad.com/
lmageJ NIH https://irnagej-nih-gov.libproxy1.nus.edu.sg/ij/index.html
STAR (Dobin et al., 2013) https://github.com/alexdobin/STAR
HTSeq (Anders et al., 2(15) N/A
R R Core Team https://www.r-project.org/
Bioconductor (Huber et al., 2015) N/A
DESeq2 (Love et al., 2014) 10.18129/B9.bioc.DESeq2
rMATS (Shen et al., 2014) http://maseq-mats.sourceforge.net/
ggsashimi (Garrido-Martin et al., 2018) https://github.com/guigolab/ggsashimi