Skip to main content
. 2020 Apr 24;11:489. doi: 10.3389/fphar.2020.00489

Figure 1.

Figure 1

Irradiation modifies voltage-dependent hERG channels in K562 cells. (A) Current tracings recorded from a control (0 Gy, upper line) and a 5 Gy-irradiated cell (lower line). Records were obtained with K-d-gluconate pipette and bath solution during a hyperpolarizing voltage step to −100 mV delivered from pre-pulses ranging from −100 to +100 mV (5 mV increments, see insert). Deactivating inward tail currents were recorded before (control), during (E4031) and after (wash-out) of the hERG1 inhibitor E4031 (1 µM). Red line indicates zero current. (B) Relationships between mean (± SE, n = 9–16) peak current density of the deactivating tail currents at −100 mV clamp voltage and the pre-pulse voltage in control (0 Gy, left) and 5 Gy-irradiated (right) K562 cells recorded as in (A) before (open circles) and during administration of E4031 (closed triangles). (C) Relationships between mean (± SE, n = 6–9) E4031-sensitive fractions of peak current density and activating pre-pulses in control (0 Gy, black circles) and 5 Gy-irradiated (red circles) as recorded in (A). * indicates p ≤ 0.05, two-tailed (Welch-corrected) t-test.