Figure 5.

The effect of berberine on extinction of cocaine-induced drug-associated behavior in CPP and IVSA extinction paradigms (A) The experimental timeline of CPP training and tests, and drug treatments (B) CPP scores of pre-test and post-test. n = 16; ***p < 0.001, compared to the pre-test (C) Extinction of cocaine-induced drug-associated behavior in a free access CPP extinction paradigm. Corn oil (1 mL/kg, i.g.) and berberine (25 mg/kg, i.g.) were administered to mice in the control and berberine treatment groups, respectively, 1 h after each extinction training session (D) Reinstatement of cocaine-induced original drug-associated memory after CPP extinction. A priming injection of saline (1 mL/kg, i.p.) and cocaine (5 mg/kg, i.p.) was administered 10 min before the reinstatement tests 1 and 2, respectively (E) The experimental timeline of cocaine IVSA training and drug treatments (F) Active nose-pokes of last 3 days of IVSA training (G) Active nose-pokes during the extinction of cocaine IVSA. Corn oil (1 mL/kg, i.g.) and berberine (25 mg/kg, i.g.) were administered 1 h after each extinction training session (H) Active and inactive nose pokes in the reinstatement of cocaine IVSA. A priming injection of saline (1 mL/kg, i.p.) and cocaine (5 mg/kg, i.p.) was administered 10 min before the reinstatement test. n = 11–12 per group. Data are expressed as mean ± SEM. IVSA, intravenous self-administration.