FIGURE 2.

Sequence comparisons of TcNudix1, RhNudix1, AtNudix1, AtNudix2, AtNudix5, AtNudix6, AtNudix11, and AtNudix15. (A) Sequence alignment of TcNudix1 and related sequences. Alignment was conducted by ClustalW alignment BioEdit. Black background indicates amino acid identity between sequences and gray background represents amino acid similarity (Threshhold of shading = 60%). The predicted plastid signal peptide of TcNudix1 is underlined. Two boxes show the consensus motifs. (B) Maximum-likelihood phylogenetic tree of sequences. RhNudix1 and AtNudix1 were reported to hydrolyze prenyl diphosphate (GPP for RhNudix1 and DMAPP and IPP for AtNudix1). AtNudix11 showed no activity with several prenyl diphosphates but its substrate has not yet been identified (Schilmiller et al., 2012; Henry et al., 2018). No biochemical characterizations of AtNudix2 and AtNudix15 have been performed (Schilmiller et al., 2012; Li J. et al., 2019; Zhou and Pichersky, 2020).