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. 2020 Apr 24;11:482. doi: 10.3389/fpls.2020.00482

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Copyright © 2020 Li, Lybrand, Xu, Zhou, Last and Pichersky.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Complete conversion of CPP to chrysanthemol by TcNudix1 and additional phosphatase(s) from pyrethrum tissues. DMAPP was incubated with TcCDS to generate CPP, followed by the addition of TcNudix1, TcNudix with crude flower protein (TcFlower¹), only crude flower protein extract, TcNudix1 with crude leaf protein (TcLeaf²), and only crude leaf protein. Chrysanthemol was detected by GC-MS. DMAPP and TcCDS with alkaline phosphatase treatment was used as positive control. Tetradecane was added as internal standard.