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. 2020 Jan 22;11:427. doi: 10.1038/s41467-019-14191-1

Fig. 5. Loss of nctA and nctB increases the cellular ergosterol content.

Fig. 5

Ergosterol levels of the wild-type (WT), the nctA null mutant (∆nctA), the nctB null mutant (∆nctB) and the nctA reconstituted isolate (nctA rec) in RPMI-1640 incubated for 24 h were determined by GC–MS. Ergosterol content of each mutant was normalised to that of the wild-type, and shown as a relative fold change. Samples were assessed in biological triplicates. P-values were calculated using one-way ANOVA: *P < 0.05; ns, P > 0.05. The error bars represent standard error of the mean. Source data are provided as a Source Data file.