FIGURE 2.

Ca²⁺ mobilization and degranulation induced by MRGPRX2 ligands is reduced by osthole in RBL-2H3 cells stably expressing MRGPRX2. RBL-2H3 cells stably expressing the MRGPRX2 receptor were treated with vehicle (0.2% DMSO in PBS) or osthole (indicated concentrations) for 30 min. (A,C,E) Ca²⁺ mobilization and (B,D,F) degranulation assays were performed following incubation with (A,B) compound 48/80 (100 ng/ml), (C,D) substance P (300 nM) or (E,F) LL-37 (3 μM). Results shown are mean ± SE of three independent experiments. Statistical significance was determined by unpaired Student’s t-test and values from the osthole-treated group was compared with the control vehicle group *p < 0.05 and **p < 0.01.