FIGURE 5.

Osthole reduces paw edema to compound 48/80 in vivo. (A) Mouse peritoneal cells were exposed to vehicle (0.2% DMSO in PBS) or osthole (72 μM) for 30 min and stimulated with the indicated concentrations of compound 48/80. Graph shows degranulation of mast cells as estimated by β-hexosaminidase release in supernatants. (B–D) Vehicle- (0.2% DMSO in PBS) or osthole- (100 mg/kg) treated C57BL/6 mice were exposed to compound 48/80, (C48/80, left paw) or PBS (right paw) and Evans blue dye was injected i.v., Mice were culled 30 min later. (B) Representative pictures of mice with dye leakage in the paws are shown. (C) The paws were excised and weighed; the dye was extracted and absorbance of the supernatant was measured at 650 nm. (D) Bar graph shows the change in paw thickness (%) of mice following compound 48/80 injection. Data shown are mean ± SE from three experiments (a total of n = 5–13 mice/group). Statistical significance was determined by unpaired Student’s t-test *p < 0.05 and **p < 0.01.