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. 2020 Apr 28;8:e9029. doi: 10.7717/peerj.9029

Figure 4. Arginine methylation influences FBL functioning.

Figure 4

(A) Detection of NOP1, FBL, GAK-FBL and GAA-FBL transcripts by RT-PCR analysis in different yeast strains coexpressing endogenous NOP1 and exogenous FBL, GAK-FBL or GAA-FBL. The primers against regions in yeast NOP1 and the human GAR and mutated GAR domains (i.e., the GAK and GAA domains) were used. (B) Coexpression of the unmethylated forms of FBL (GAK-FBL and GAA-FBL) did not influence the survival of the GAL-NOP1 cells (Galactose). Expression of the full-length human FBL but not GAK-FBL or GAA-FBL complemented NOP1 repression in S. cerevisiae (Glucose). (C) Cell viability of the yeast cells expressing FBL, GAK-FBL, GAA-FBL, or the empty vector p416GPD after growth on glucose medium. Colony forming units (CFUs) were counted for each yeast strain. Yeast survival was estimated as the ratio of the cells on the glucose medium to the ratio of the cells on the galactose medium (CFU D/CFU GAL).