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ICS II enhanced cGMP level and PKG activity (A) cGMP protein expression was detected by ELISA kit. (B) PKG activity was determined by PKG activity kit. PKG-specific inhibitor Rp-8-Br-cGMP (Rp.) abolished the beneficial effects of ICS II. (C) Cell viability tested by MTT assay. (D) LDH release was detected using an LDH release assay kit. **P < 0.01 versus control group; #P < 0.05, ##P < 0.01 versus OGD/R group, &P < 0.01 versus OGD/R+ ICS II 50 group.
