Figure 4.

PPBs of plasma protein solutions spiked with VRC in vitro were grouped according to protein concentration. ALB binding rate of samples in higher C_t group (25,000 ng/ml) with higher protein concentration was significantly higher (A, 50 vs. 30 g/L, p < 0.01). AAG binding rate of samples with higher protein concentration had a significant higher PPB in both two C_t groups (B, C_t=400 ng/ml, [0.01 vs. 1 g/L, p = 0.001]; C_t=25,000 ng/ml, [0.01 vs. 1 g/L, p < 0.01]); GLB binding rate was without this characteristic in either lower C_t group or higher C_t group (C). Plasma protein mixtures spiked with a higher concentration of protein (C_p=81 g/L) had a significant higher PPB than the lower group (C_p=40.01 g/L) in both two different C_t groups (D, C_t=400 ng/ml, [40.01 vs. 81 g/L, p < 0.01]; C_t=25,000 ng/ml, [40.01 vs. 81 g/L, p < 0.01]). There was no significant difference between lower ALB concentration and higher ALB concentration in either lower C_t group or higher C_t group (E). In a higher C_t (25,000 ng/ml), C_u of a lower AAG concentration was significantly higher than samples with higher AAG concentration (F, 0.01 vs. 1 g/L, p < 0.01). In a higher C_t (25,000 ng/ml), C_u of in vitro samples with a lower GLB concentration (10 g/L) was significantly higher than samples with higher GLB concentration (G, 30 g/L, p < 0.01). In a lower C_t (400 ng/ml), C_u of a lower plasma protein concentration was significantly higher than sample with a higher plasma protein concentration (H, 40.01 vs. 81 g/L, p < 0.01).