Table 2.

Protein binding rates among different sample types.

Samples		n	PPB% (Mean ± SD)	p
In vivo samples		79	69.5 ± 6.2	vs. healthy human plasma, p < 0.01;
				MIX, p < 0.01;
				ALB, p < 0.01;
				ACG, p < 0.01;
				GLB, p < 0.01;
In vitro samples	VRC spiked with healthy human blank plasma	80	58.0 ± 4.7	vs. MIX, p = 0.066
				ALB, p < 0.01;
				ACG, p < 0.01;
				GLB, p < 0.01;
	VRC spiked with protein MIX (ALB+AAG+GLB)	24	56.1 ± 4.6	vs. ALB, p < 0.01;
				ACG, p < 0.01;
				GLB, p < 0.01;
	VRC spiked with human ALB	42	42.9 ± 6.3	vs. ACG, p = 0.020;
				GLB, p < 0.01;
	VRC spiked with human AAG	36	46.5 ± 7.6	vs. GLB, p < 0.01
	VRC spiked with human GLB	24	37.8 ± 4.1

VRC, voriconazole; ALB, albumin; AAG, α1-acid glycoprotein; GLB, globulin; PPB, plasma protein binding rate.