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. Author manuscript; available in PMC: 2020 Sep 30.
Published in final edited form as: Nat Cell Biol. 2020 Mar 30;22(4):476–486. doi: 10.1038/s41556-020-0496-x

Fig. 5. Aberrant expression of SLC7A11 sensitizes cancer cells to GLUT inhibition.

Fig. 5.

a, Glucose uptake levels were measured in 786-O cells treated with 2 μM KL-11743 or BAY-876. b, Measurement of NADP+/NADPH ratios in EV and SLC7A11-overexpressing-786-O cells treated with KL-11743, BAY-876, or cultured in glucose free medium. c, Cell death was measured by PI staining in different cancer cell lines treated with 2 μM KL-11743 or BAY-876. d, e, Representative phase-contrast images and cell death of EV and SLC7A11-overexpressing 786-O cells treated with 2 μM KL-11743 (d) or BAY-876 (e). The experiment was repeated four times, independently, with similar results. f, g, GLUT inhibition-induced cell death in control (sgCtrl) and SLC7A11 knockout (sgSLC7A11) UMRC6 (f) or NCI-H226 (g) cells were measured by PI staining. h, Measurement intracellular levels of cystine in EV and SLC7A11-overexpressing-786-O cells treated with KL-11743, BAY-876, or cultured in glucose free medium. i, j, Cell death was measured by PI staining in 786-O (i) or ACHN (j) cells with SLC7A11 and/or G6PD overexpression treated with 2 μM KL-11743 or BAY-876. All p values were calculated using two-tailed unpaired Student’s t-test. Detailed statistical tests are described in the Methods. All error bars are mean± s.d., n=3 independent experiments. All scale bars=100 μm. Numeral data are provided in Statistics Source Data Fig. 5.