Table 1.

Time considerations

Basic protocol	Steps	Hands-on-time	Total experiment time	Stopping point
Basic Protocol1& 2		−7hr	~ 4days	Yes
Identification of zebrafish	Prepare Cas9 plasmid	−2hr
CRISPR/Cas9 target site	Identify CRISPR/Cas9 target site	−1hr
	Making sgRNA	−4hr
Basic Protocol 3		−5hr	~ 2 days	No
Zebrfish microinjections and scoring phenotype	Set up matings	−1hr
	Inject next day morning into 1-cell embryos	− 2hr
	Scoring F0 phenotype at 48hrs	−2hr
Basic Protocol 4	Identifying mutations in CRISPR/Cas9 injected embryos by HRMA	−8hr	~ 2 days	No
Basic Protocol 5	Identification of mutation in CRISPR/Cas9 injected embryos by Mobility Shift Assay	−6hr	~1 day	Yes