| Optimal Conditions | Range |
| 62°C Tm | 58-64°C |
| ~80 bp | 65-450 bp amplicon |
| 50% GC | 40-70% GC content |
| 20 bp | 19-25 bp primer length |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.