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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Nat Plants. 2019 Sep 16;5(10):1057–1065. doi: 10.1038/s41477-019-0511-z

Fig. 2. Overexpression of CYP72A9 results in a dwarf phenotype and decreases endogenous GA4 levels.

Fig. 2

a. GFPPS-sesterTPS-P450 gene cluster and phenotypes of Arabidopsis with increased expression level of each AtCYP72 (CYP72A7, A8, A9, A10, A11, A13, A14, and A15). All plants were grown under the same growth conditions and images were taken at 24 days after germination. This experiment was repeated at least three times with similar results. E.V., empty vector; Scale bar = 5 cm.

b. Exogenous applied GA3 rescues the growth of ga1-t mutant and CYP72A9-overexpressing Arabidopsis. All one-week-old seedlings, which grown on ½ MS agar medium, were transferred onto ½ MS agar medium and grown for another week with or without 2 μM bioactive GA3. This experiment was repeated three times with similar results. WT, wild type. Scale bar = 1 cm.

c. Profile of endogenous GAs in WT and two independent CYP72A9-overexpressing lines. The GA levels in the rosette leaves of 4-week-old Arabidopsis are presented as the means ± SDs (n = 3 biologically independent samples). **, significant difference from WT (P < 0.01; two-tailed Student’s t-test). N.D., not detectable; N.Q., detected, but not quantifiable due to low abundance.