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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Nat Protoc. 2019 Dec 20;15(1):161–180. doi: 10.1038/s41596-019-0260-5
Time from start (min:s) Duration (min:s) Flow (nL/min) % Buffer B
00:00 NA 300 3
60:00 60:00 300 30
70:00 10:00 300 50
71:00 01:00 300 95
75:00 04:00 300 95

NA, not applicable.