Table 2.
Comparisons of 28S-gB-CAR-T, BBL-gB-CAR-T, and irrelevant control CD19-CAR-T cells after five sequential cocultures with mesenchymal stem cells/human cytomegalovirus
| 28S |
28L |
BBL |
CD19 |
p |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mean (SD) | Mean (SD) | Mean (SD) | Mean (SD) | 28S × 28L | 28S × BBL | 28S × CD19 | 28L × BBL | 28L × CD19 | BBL × CD19 | |
| After second iteration | ||||||||||
| Number of T cells | 5.24E+05 (9.80E+04) | 6.08E+05 (6.63E+04) | 4.25E+05 (1.62E+05) | 1.50E+05 (2.87E+04) | ns | ns | *** | * | *** | *** |
| IFN-γ (pg/mL) | 2.02E+03 (1.12E+03) | 6.24E+03 (2.01E+03) | 3.65E+03 (5.98E+02) | 0 (0) | *** | * | ** | *** | *** | *** |
| % Viable MSC | 3.12E+01 (1.27E+01) | 2.73E+01 (1.06E+01) | 3.03E+01 (1.13E+01) | 4.97E+01 (1.46E+01) | ns | ns | ns | ns | ns | ns |
| After last iteration | ||||||||||
| % CAR+/CD4+ | 9.28E+01 (3.2E-01) | 9.18E+01 (2.31E+00) | 8.23E+01 (8.07E+00) | 7.08E+01 (4.01E+00) | ns | * | *** | ns | *** | * |
| % CAR+/CD8+ | 9.45E+01 (8.0E-01) | 8.97E+01 (1.28E+00) | 7.52E+01 (8.16E+00) | 7.22E+01 (5.05E+00) | ns | *** | *** | ** | *** | ns |
| % PD-1+/CAR+/CD4+ | 2.80E+01 (6.66E+00) | 2.61E+01 (2.92E+00) | 1.81E+01 (7.98E+00) | 3.76E+00 (7.0E-01) | ns | ns | *** | ns | *** | * |
| % PD-1+/CAR+/CD8+ | 4.18E+01 (7.49E+00) | 3.48E+01 (5.81E+00) | 6.59E+00 (3.09E+00) | 8.56E+00 (5.31E+00) | ns | *** | *** | *** | *** | ns |
The following groups were compared: 28S-gB-CAR-T, 28L-gB-CAR-T, BBL-gB-CAR-T, and the irrelevant reference control CD19-CAR-T cells. CAR-T cells were generated from a single AB donor. Cocultures were performed in independent triplicate experiments, and for each experiment, MSCs derived from a different donor were used. Results pertaining to T cell numbers, IFN-γ detection, and remaining viable MSCs are shown for cocultures of CAR-T cells with MSC/HCMV after the second iterations. Results pertaining to the frequencies of CD4+ and CD8+ CAR-T cells and PD-1+CD4+ CAR-T and PD-1+CD8+CAR-T are shown for the cells recovered after the last iteration. Statistical significance was determined by two-way ANOVA and Bonferroni's post hoc tests between the indicated groups.
p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.