Table 2.
MAD7 gene editing efficiency in mouse and rat embryos
| Targeting gRNA | MAD7 form | Donor | No. of injected zygotes | No. of surviving and transferred | No. of ∼E14 embryos (%)a | No. of newborns | No. of mutations (%)b | No. of integrations (%)b |
|---|---|---|---|---|---|---|---|---|
| Mouse Rosa26 #1 | mRNA | — | 90 | ND | 42 | — | 25 (60) | - |
| Mouse Rosa26 #1 | mRNA | ssODN | 55 | 50 | 10 (20) | — | 3 (30) | 2 (20) |
| Mouse Rosa26 #1 | Protein | ssODN | 80 | 60 | 5 (8) | — | 3 (60) | 1 (20) |
| Mouse Rosa26 #1 | mRNA | ssODN | 100 | 48 | — | 3 | 1 (33) | 1 (33) |
| Mouse Rosa26 #2 | mRNA + Protein | Plasmid | 528 | 296 | — | 48 | 39 (81) | 1 (2) |
| Rat Calb2 #1 | mRNA + Protein | Plasmid | 351 | 273 | — | 49 | 10 (20) | 12 (24) |
a
% calculated as number of E14 embryos divided by the number of surviving and transferred embryos.
b
% calculated as number of mutations or integrations divided by the number of E14 embryos or newborns.
E14, embryonic day 14.