Fig 6. Effect of BBR on PA and LPS-induced ERK activation in RAW264.7 macrophages.

RAW264.7 cells are pre-treated with BBR (0, 1.25, 2.5, 5, 10 μM) for 1 h, then treated with PA(0.25mM) and LPS (25 ng/mL) for 6 h. Total cell lysates were prepared for Western blot analysis as described under Materials and Methods. Values are mean ±S.E. of three independent experiments. Statistical significance relative to vehicle control, *p<0.05; relative to PA+LPS, ^#p<0.05, ^##p<0.01. A. Representative immunoblots of phospho(p)-ERK and total (T)-ERK; B. The relative protein levels of p-ERK/total ERK.