Figure 3.

Mutation in ER-resident UDP-glucose: glycoprotein glucosyltransferase (UGGT) induces the mislocalization of GFP-KOR1. (a) Schematic diagram of the UGGT locus (open boxes represent exons). Positions for mutations are indicated. (b) Subcellular localization of GFP-KOR1 in the 2C11 mutant and F1 lines obtained from genetic crosses between 2C11 and indicated uggt T-DNA insertion lines (uggt-11 and uggt-12). The scale bar indicates 10 mm. (c) Confocal microscopy images of GFP-KOR1 in 7-day-old 2C11 plants, uggt-11, uggt-12, and F1 lines grown on 1 x MS, 3% sucrose medium. The white arrowheads indicate GFP signals at the TP. The scale bars indicate 10 µm.