Figure 2.
Heterologous expression siR109944 affects Arabidopsis auxin-related miRNA expression. (a) The expression levels of auxin-related miRNAs (miR160, miR167, and miR165/166) in Col-0 WT and siR109944 OE plants by northern blot. Values below each section represent the relative abundance (RA) of miRNA normalized to U6. (b) Quantitative PCR analysis of the relative expression of the miR160 target gene ARF10, miR167 target gene ARF6, and miR165/166 target genes PHN and PHB in Col-0 WT and siR109944 OE plants. The internal reference was AtActin. The experiments were repeated three times with similar results. Asterisks indicate significant differences (*P < .05).