Figure 1.

(a) The number of infection threads observed in the WT, cle-rs1 and cle-rs2 mutants and in the cle-rs1 cle-rs2 double mutant with 0 or 10 mM KNO₃ at 7 days after inoculation (dai) with Mesorhizobium loti MAFF303099 constitutively expressing DsRed (n = 10–12 plants). Stable cle-rs mutants were previously created by the CRISPR-Cas9 genome editing system.²¹ Columns with the same lower-case letter indicate no significant difference (Tukey’s test, P < .05). (b) The number of infection threads in the WT and daphne mutants with 0 or 10 mM KNO₃ at 5 dai with Mesorhizobium loti MAFF303099 constitutively expressing DsRed (n = 10–12 plants). *P < .05 by Student’s t-test. Error bars indicate SE.