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Comparison of BAS (a-c) and CYP716A (d-g) proteins for β-amyrin and oleanolic acid production. (a, d) OD600 at end of culture (grey). (b, e) Titres (mg/L) of β-amyrin (red), erythrodiol (yellow) and oleanolic acid (cyan). (f) Oleanolic aldehyde peak areas obtained from GC/QTOF-MS analysis. (c, g) β-Amyrin (dark red) and oleanolic acid (dark cyan) productivities (mg/L/OD600). None, empty vector strain MD-N1 in a-c and strain expressing AaBAS only in d-g. Mean ± SD for n = 3 is presented.
