Figure 2. Ephrin-A1-deficient hosts have reduced cancer cell lung colonization.

( A) Representative image of bioluminescence signal in WT and KO littermates several hours after tail vein injection of 1×10 ⁵ 4T1-GFP-luciferase cells. ( B) Quantification of bioluminescence signal in WT, +/-, and KO littermates. ( C) Representative images of GFP+ surface lung metastases in WT and KO littermates 17 days after tail vein injection. ( D) Blinded quantification of GFP+ lung metastases in WT, +/-, and KO littermates and percentages of GFP+ 4T1 cells in the lung from flow cytometry analysis. ( E) Representative H&E staining of left lung lobes from WT and KO littermates and blinded quantification of metastatic foci per lung section. Scale bar: 200 µm. Data shown are averages ± SD with each data point representing an individual mouse ( n=4–9 mice per group). * p<0.05, ** p<0.01 (unpaired Mann-Whitney U-test for comparisons between two groups, Kruskal-Wallis H-test with post-hoc unpaired Mann-Whitney U-test for comparisons between three groups).