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. 2020 May 14;9:217. Originally published 2020 Mar 30. [Version 2] doi: 10.12688/f1000research.22689.2

Figure 4. Tumor vascularity and pericyte coverage are not significantly different in ephrin-A1-deficient hosts.

Figure 4.

( A) Representative images of CD31 (red), αSMA (green), and DAPI (blue) staining on cryosections of 4T1 primary tumors harvested from WT and KO littermates at day 14 post-implantation. Scale bar: 100 µm. ( B) Quantification of CD31+ area and integrated intensity in arbitrary units (au) per high power field (hpf) of view. ( C) Quantification of αSMA colocalization with CD31 as a percentage of αSMA+ over CD31+ stained area and integrated density. Data shown are averages ± SD with each data point representing an individual mouse ( n=3–7 mice per group).