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. 2020 May 14;9:217. Originally published 2020 Mar 30. [Version 2] doi: 10.12688/f1000research.22689.2

Figure 5. Ephrin-A1-deficient lung microenvironment provides a less favorable metastatic niche.

Figure 5.

( A) Quantification of GFP+ 4T1 cells and ( B) CD31+ endothelial cells by flow cytometry in perfused lungs harvested from WT and KO littermates 24 hours post-tail vein injection. ( C) Percentage of GFP+ 4T1 cells in perfused WT and KO lungs that still contained CellTrace Violet dye, indicating reduced proliferation. ( D) Representative images of PCNA staining on FFPE sections of tumor-bearing lungs from WT and KO littermates 17 days after tail vein injection of 4T1-GFP-luciferase cells. Higher magnification images and blinded quantification of PCNA+ tumor cell nuclei shown. Scale bar: 50 µm. Data shown are averages ± SD with each data point representing an individual mouse ( n=3–6 mice per group). * p<0.05 (unpaired Mann-Whitney U-test).