Table 1. Antibodies used in flow cytometry analysis.
| Antibody target | Manufacturer | Catalog # | Fluorophore | Dilution | RRID |
|---|---|---|---|---|---|
| MHCII I-E/A | Tonbo Biosciences | 75-5321 | V450 | 1/250 | AB_2621965 |
| CD8a | BD Biosciences | 560469 | V450 | 1/250 | AB_1645281 |
| CD11b | Tonbo Biosciences | 35-0112 | FITC | 1/250 | AB_2621676 |
| CD62L | Tonbo Biosciences | 35-0621 | FITC | 1/100 | AB_2621697 |
| CD44 | Tonbo Biosciences | 50-0441 | PE | 1/5000 | AB_2621762 |
| CTLA-4 | BD Biosciences | 561718 | PE | 1/250 | AB_10895585 |
| CD31 | BD Biosciences | 561073 | PE | 1/750 | AB_10563931 |
| CD4 | Biolegend | 100516 | APC | 1/1000 | AB_312719 |
| Ly6C | BD Biosciences | 560595 | APC | 1/500 | AB_1727554 |
| FoxP3 | eBiosciences | 50-5773-82 | e660 | 1/100 | AB_11218868 |
| F4/80 | eBiosciences | 45-4801-82 | PerCP-Cy5.5 | 1/250 | AB_914345 |
| CD3e | Tonbo Biosciences | 65-0031 | PerCP-Cy5.5 | 1/250 | AB_2621872 |
| Ly6G (Gr1) | Tonbo Biosciences | 80-5931 | rF710 | 1/1000 | AB_2621999 |
| CD8a | Tonbo Biosciences | 80-0081 | rF710 | 1/500 | AB_2621977 |
| PD-1 | BD Biosciences | 565815 | APC-R700 | 1/500 | AB_2739366 |
| CD45 | Biolegend | 103109 | PE-Cy5 | 1/5000 | AB_312974 |
| CD4 | Tonbo Biosciences | 55-0041 | PE-Cy5 | 1/2500 | AB_2621816 |
| CD69 | BD Biosciences | 552879 | PE-Cy5 | 1/1000 | AB_394508 |
| CD11b | Tonbo Biosciences | 55-0112 | PE-Cy5 | 1/5000 | AB_2621818 |
| EpCAM | Biolegend | 118215 | PE-Cy7 | 1/750 | AB_1236477 |
| CD11c | BD Biosciences | 561022 | PE-Cy7 | 1/500 | AB_2033997 |
| Ly6C | eBiosciences | 25-5932-80 | PE-Cy7 | 1/1000 | AB_2573502 |
| CD25 | Tonbo Biosciences | 60-0251 | PE-Cy7 | 1/500 | AB_2621843 |
| CD11c | Biolegend | 117323 | APC-Cy7 | 1/500 | AB_830646 |
| CD45 | BD Biosciences | 557659 | APC-Cy7 | 1/500 | AB_396774 |