Table 2. Gating strategy used in flow cytometry analysis.

Cell population	Gating strategy
CD8 T cells	CD45+,CD3e+,CD4-,CD8a+
CD4 T cells	CD45+,CD3e+,CD4+,CD8a-
Tregs	CD45+,CD3e+,CD4+,CD8a-,CD25+,FoxP3+
Monocytes	CD45+,CD11b+,Ly6G-,Ly6C+,F4/80-
Macrophages	CD45+,CD11b+,Ly6G-,Ly6C-,F4/80+
Granulocytes	CD45+,CD11b+,Ly6G+,Ly6C-/+,F4/80-
Dendritic cells	CD45+,CD11c+,MHCII+,F4/80-
Endothelial cells	CD45-,GFP-,EpCAM-,CD31+
4T1 cells	CD45-,GFP+,CD31-