Table 2.
Detailed information about the primers used in PCR and reverse-transcription PCR
| Virus1 | Target gene2 | Polarity | Primer sequence (5′ to 3′) | Size of amplicon (bp) | Reference |
|---|---|---|---|---|---|
| Astrovirus | ORF1b | Forward | TGGTGGTGYTTYCTCAARA | 601 | Tang et al., 2005a |
| Reverse | GYCKGTCATCMCCRTARCA | ||||
| CAstV | ORF1b | Forward | GAYCARCGAATGCGRAGRTTG | 362 | Day et al., 2007 |
| Reverse | TCAGTGGAAGTGGGKARTCTA | ||||
| ANV | ORF1b | Forward | GYTGGGCGCYTCYTTTGAYAC | 473 | Day et al., 2007 |
| Reverse | CRTTTGCCCKRTARTCTTTRT | ||||
| ChPV | NS | Forward | TTCTAATAACGATATCACTCAAGTTTC | 561 | Zsak et al., 2009 |
| Reverse | TTTGCGCTTGCGGTGAAGTCTGGCTCG | ||||
| IBV | S1 | Forward | AGGAATGGTAAGTTRCTRGTWAGA | 620 to 640 | Mase et al., 2004 |
| Reverse | GCGCAGTACCRTTRAYAAAATAAGC | ||||
| Forward | TGAAAACTGA ACAAAAGAC | Lee et al., 2000 | |||
| 3′ UTR | Forward | GAGAGGAACAATGCACAGC | 351 | Mardani et al., 2006 | |
| Reverse | CATTTCCCTGGCGATAGAC | ||||
| AvRV | VP6 | Forward | GGCTTTTAAACGAAGTCTTC | 1,350 | Ito et al., 1995 |
| Reverse | GGTCACATCCTCTCACT | ||||
| NSP4 | Forward | GGG CGT GCG GAA AGA TGG AGA AC | 630 | Day et al., 2007 | |
| Reverse | GGG GTT GGG GTA CCA GGG ATT AA | ||||
| ARV | S4 | Forward | GTG CGT GTT GGA GTT TCC CG | 1,120 | Pantin-Jackwood et al., 2008 |
| Reverse | TAC GCC ATC CTA GCT GGA | ||||
| FAdV | Hexon | Forward | TGGAC ATGGGGGCGACCTA | 1,219 | Raue and Hess, 1998 |
| Reverse | AAGGG ATTGACGTTGTCCA |
1
CAstV = chicken astrovirus; ANV = avian nephritis virus; ChPV = chicken parvovirus; IBV = infectious bronchitis virus; AvRV = avian rotavirus, ARV = avian reovirus; FAdV = fowl adenovirus.
2
ORF1b = open reading frame 1b; NS = nonstructural gene; S1 = spike 1 gene; 3′ UTR = 3′ untranslated region; VP6 = viral structural protein 6; NSP4 = nonstructural protein 4; S4 = small segment 4.