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Site-Specific Photo-Crosslinking Strategy for Studying IFITM3-Interacting Proteins in Live Cells
The amber suppression technology mediates site-specific incorporation of the diazirine-containing DiZPK into IFITM3, which enables in-cell photo-crosslinking with interacting proteins. The photo-crosslinked complexes were analyzed by western blot and identified by quantitative proteomics after IP.
