Skip to main content
. 2020 Apr 2;27(5):571–585.e6. doi: 10.1016/j.chembiol.2020.03.004

Figure 2.

Figure 2

DiZPK Is Incorporated into IFITM3 via Amber Suppression and Enables Photo-Crosslinking in Live Cells

(A) Domain structure of IFITM3 protein showing amino acid residues mutated to amber codons and locations of lysine and cysteine residues.

(B) Western blot analysis of HEK293T cells transfected with HA-IFITM3-TAG mutants in the absence or presence of DiZPK.

(C) Western blot analysis of HEK293T cells transfected with HA-IFITM3-TAG mutants in the presence of DiZPK with or without UV irradiation. High-molecular-weight bands that appeared in UV-irradiated samples indicate putative photo-crosslinked IFITM3 complexes.