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. 2020 Apr 2;27(5):571–585.e6. doi: 10.1016/j.chembiol.2020.03.004

Figure 6.

Figure 6

Depletion of VCP or UBXD1 Leads to Accumulation of IFITM3 and Delayed Turnover

(A) Analysis of endogenous IFITM3 levels upon VCP or UBXD1 knockdown. HeLa cells were transfected with indicated siRNA for 72 h and lysed for western blot analysis.

(B) Quantification of non-ubiquitinated IFITM3, VCP, and UBXD1 levels shown in (A). Data are represented as mean ± SD, n = 3. ∗∗∗∗p < 0.0001 calculated by two-way ANOVA test.

(C) Analysis of endogenous IFITM3 turnover upon VCP or UBXD1 knockdown. HeLa cells were transfected with indicated siRNA for 72 h, treated with CHX (25 μg/mL) for the indicated times, and lysed for western blot analysis.

(D) Quantification of IFITM3 levels normalized to tubulin levels shown in (C). Data are represented as mean ± SD, n = 3.