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. Author manuscript; available in PMC: 2020 Sep 26.
Published in final edited form as: Nat Microbiol. 2020 Mar 26;5(4):620–629. doi: 10.1038/s41564-020-0692-2

Extended Data Fig. 1. Schematic of acr loci and lethal self-genome cleavage assay.

Extended Data Fig. 1

a, Full schematic of acr loci with relevant neighboring genes displayed. b, Schematic of SpyCas9 in P. aeruginosa programmed to cause lethal self-genome cleavage to assess bacterial survival in the presence of AcrIIA proteins. CRISPR strength is determined by titrating levels of IPTG, which induces expression of sgRNA targeting the chromosomal phzM gene from a multicopy plasmid. OD600 measurements are represented as the mean of three biological replicates ± SD.