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. 2020 May 1;6(18):eaaz0952. doi: 10.1126/sciadv.aaz0952

Fig. 1. Cellular uptake of IONPs and IONP-triggered cellular modification.

Fig. 1

(A) Schematic illustration and TEM image of IONPs. PEG, polyethylene glycol. (B) Fluorescent images of IONP (red) uptake by MSCs 24 hours after IONP treatment [green, cell membrane; and blue, 4′,6-diamidino-2-phenylindole (DAPI)]. Scale bars, 100 μm. (C) The cytotoxicity of IONPs 2, 4, 7, 10, and 14 days after IONP treatment to MSCs, as evaluated by CCK assay (n = 4 per group; NS, no significant difference). (D) Relative mRNA expression levels of various cardiac repair–favorable genes in MSC and IONP-MSC, as evaluated by qRT-PCR (n = 4 per group). (E) Schematic illustration of the IONP-mediated intracellular signaling cascades in MSCs and Western blot analysis for the intracellular signaling cascade molecules and associated therapeutic molecules (n = 3 per group). *P < 0.05 by two-tailed t test. All values are means ± SD.