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. 2020 May 1;6(18):eaaz0952. doi: 10.1126/sciadv.aaz0952

Fig. 6. Enhanced blood vessel density, the improvement of left ventricular remodeling, and the promotion of cardiac function recovery.

Fig. 6

(A) Representative images of functional blood vessels visualized by perfusion of isolectin B4 and immunostaining for cTnT (CM marker) in the infarct zone and border zone 4 weeks after injection and the quantitative data (n = 5 animals per group). Scale bars, 50 μm. (B) Representative Masson’s trichrome–stained infarcted hearts four weeks after injection (blue, scar tissue; red, viable myocardium). (C) The fibrosis area, scar size, viable myocardium area, and thickness of the left ventricular (LV) wall 4 weeks after injection (n = 5 animals per group). *P < 0.05, using one-way ANOVA, followed by post hoc Bonferroni test. (D) Representative M-mode images 1 and 4 weeks after treatment. Yellow arrows between wall motions indicate left ventricular dimension. Distance between yellow arrows and arrowheads in top and bottom area indicates left ventricular wall thickness and left ventricular posterior wall thickness, respectively. (E and F) Ejection fraction and fractional shortening at various time points (n = 5 animals per group). *P < 0.05 versus NT, #P < 0.05 versus IONP, †P < 0.05 versus N-NV, and ‡P < 0.05 versus IONP-NV, using two-way ANOVA, followed by post hoc Bonferroni test. (G and H) Left ventricular end diastolic dimension (LVIDd) and left ventricular end systolic dimension (LVIDs) at various time points (n = 5 animals per group). (I and J) Posterior wall thickness and septal wall thickness at various time points (n = 5 animals per group). *P < 0.05, using two-way ANOVA, followed by post hoc Bonferroni test. The group names in (E to J) are referred to Fig. 6 (C and D). All values are means ± SD.