Table 1.

Comparison of different cardiac models generated with cells at different time points and submitted to different environments

Settings culture	Culture 2D	3D Tissues				Adult cardiomyocytes
		Late cells	Early Cells
		Intensity stimulation	Control	Constant stimulation	Intensity stimulation
CELL MORPHOLOGY
Cell size	+	++	+	++	+++	+++
T-tubules	-	-	-	-	++	+++
Intercalated discs	-	-	-	+	++	+++
Sarcomere length	++	-	-	+	++	+++
Membrane potential	+	+	+	++	+++	+++
METABOLISM
Primary metabolic function	glycolysis	glycolysis	glycolysis	glycolysis	fatty acid oxidation	fatty acid oxidation
Mitochondria density	+	+	+	+	++	+++
PHYSIOLOGY
Force- frequency relationship	-	-	-	-	+	+
Calcium- induced response	+	+	+	++	+++	+++
Isoproterenol response					lonotropic, lusitropic chonotropic	lonotropic, lusitropic chonotropic
GENE EXPRESSION
Gene Expression Profile	Fetal-like	Fetal-like	Fetal-like	Fetal-like	Adult-like	Adult