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. Author manuscript; available in PMC: 2020 Sep 6.
Published in final edited form as: J Proteome Res. 2019 Aug 28;18(9):3447–3460. doi: 10.1021/acs.jproteome.9b00342

Figure 1.

Figure 1.

TGFβ induced EMT activates unfolded proteins response and protein N-glycosylation. hSAECs were treated with TGFβ for 14 d to induce stable mesenchymal transition. (A) 2D annotation enrichment based on the protein and mRNA ratios (the proteome and transcriptome data used in the figure were published previously by our group1,2). (B) Box-plots of mRNA expression of the entries within representative categories identified that were up-regulated in EMT state (e.g., extracellular matrix organization, unfolded protein response, and protein N-linked glycosylation) and down-regulation in EMT (e.g., desmosome). (C) Box-plots of the protein expression of the entries within representative categories identified as significantly altered by the 2D annotation enrichment analysis. (D) Lectin staining of glycoproteins in hSAECs and EMT-hSAECs. The fixed hSAECs (left) and EMT-hSAECs (right) were stained with DAPI for nuclei (blue) and lectin for glycoproteins (green) (magnified ×63).