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. Author manuscript; available in PMC: 2021 May 2.
Published in final edited form as: J Thorac Cardiovasc Surg. 2019 Nov 2;161(1):e81–e90. doi: 10.1016/j.jtcvs.2019.09.188

Figure 1.

Figure 1.

Flow Cytometry gating for DN T cells. Cells isolated from murine lungs were cultured in 5% fetal bovine serum in RPMI medium with 100 mg of Collagenase D for 30 minutes. Cells were strained and lymphocytes were isolated with percoll extraction. Lymphocytes were stained with CD45 (APC-Cy7), TCR αβ (BV421), CD4+ (APC), CD8+ (FITC) antibodies and analyzed through LSR-II flow cytometry. The lymphocyte population was isolated from SSC-A and FSC-A. CD45+ TCR+ subpopulation was selected to visualize CD4+, CD8+, Double-Positive (CD4+CD8+) and Double-Negative (CD4−CD8−) T cells. Double-Negative T cells are represented as the population within the red circle.