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. Author manuscript; available in PMC: 2021 May 2.
Published in final edited form as: J Thorac Cardiovasc Surg. 2019 Nov 2;161(1):e81–e90. doi: 10.1016/j.jtcvs.2019.09.188

Figure 6.

Figure 6.

DN T cell transfer attenuates lung IRI. For T cell adoptive transfer, highly purified (>97%) total T cells were isolated from lymph nodes of gld mice. 2.0 X 106 of either unconventional DN T cells or conventional CD4+ and CD8+ T cells were transferred into separate groups of mice (n=5 per group), 12 hours before lung IRI. (A) Mice preoperatively treated with DN T cells and then underwent lung IRI, exhibited a significant reduction in interstitial edema and Evans blue dye permeability when compared to mice who only received conventional T cells. (C) Immunoblotting analysis also showed a significant decrease in cleaved caspase-3 in mice who received DN T cells. There was no significant difference between mice that only underwent IRI and mice who received conventional T cells prior to IRI.