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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Cryobiology. 2019 Nov 18;92:67–75. doi: 10.1016/j.cryobiol.2019.11.004

Figure 1.

Figure 1.

Deep-supercooling (DSC) of solutions and cell suspensions via surface sealing by paraffin oil (PO). (A) Representative image of cell suspensions without (Suspension) or with (Suspension + PO) surface sealing at −13 °C. 1 ml cell suspension of 1 million human adipose-derived stem cells (hADSCs) was stored in each polystyrene tube for 7 days. The storage solution is the basic University of Wisconsin solution (UW solution). (B) Cumulative freezing frequency (ff = number of frozen samples/total number of initial samples) for 1 ml UW solution or cell suspensions with or without surface sealing over 7 days. Number of independent experiments n = 5, number of tested samples for each case N = 50. Error bars represent standard deviations.