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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Cryobiology. 2019 Nov 18;92:67–75. doi: 10.1016/j.cryobiol.2019.11.004

Figure 2.

Figure 2.

DSC preservation of hADSCs enabled by oil sealing. (A) Phase and fluorescence micrographs of cell viability staining post 7 - day storage at various temperatures. Three typical storage solutions, ADSC media, UW, and UW supplemented with 5% (w/v) 35 Kd polyethylene glycol (PEG) and 0.2 M 3-O-methyl glucose (3-OMG) (UW + PEG + 3-OMG), were tested. Live cells were stained green by calcein AM while dead cells were red by ethidium homodimer. (B) Quantitative cell viabilities post 7 - day storage under various scenarios. Number of independent experiments n = 3.