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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Cryobiology. 2019 Nov 18;92:67–75. doi: 10.1016/j.cryobiol.2019.11.004

Figure 4.

Figure 4.

Stem cell genes expressions and immunofluorescence staining of surface protein markers. (A) Gene expression levels examined by RT-PCR. Four stem cell genes OCT 4, SOX2, KLF4 and NANOG for ADSCs were examined. Number of independent experiments n = 3, #: p > 0.1. (B) Immunofluorescence staining for stem cell surface markers. Both positive (CD 44+) and negative (CD 31 −) surface markers were examined for fresh control and DSC preserved cells at −16 °C for 7 days.