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. Author manuscript; available in PMC: 2020 Oct 13.
Published in final edited form as: Nat Neurosci. 2020 Apr 13;23(5):638–650. doi: 10.1038/s41593-020-0617-7

Extended Data Fig. 3. Anterograde tracing of LHB GAD2 neuron projections.

Extended Data Fig. 3

a, Schematic of surgical manipulations for anterograde tracing of GAD2 LHb neurons. b, Representative image of viral infection in GAD2 LHb neurons. Experimental images were obtained from 3 biologically independent mice, three slices per mouse, with similar results obtained. c, Representative image of the interpeduncular nucleus (IPN) and ventral tegmental area (VTA) in mice expressing eGFP in GAD2 LHb neurons. Experimental images were obtained from 3 biologically independent mice, three slices per mouse, with similar results obtained. d, Representative image of the rostromedial tegmental nucleus (RMTg) in mice expressing eGFP in GAD2 LHb neurons. Experimental images were obtained from 3 biologically independent mice, three slices per mouse, with similar results obtained. e, Representative image of the RMTg and anterior dorsal and median raphe nuclei (DRN and MRN) in mice expressing eGFP in GAD2 LHb neurons. Experimental images were obtained from 3 biologically independent mice, three slices per mouse, with similar results obtained. f, Schematic of surgical manipulations for non-conditional anterograde tracing of LHb neurons. g, Representative image of viral infection in LHb neurons. Experimental images were obtained from 3 biologically independent mice, three slices per mouse, with similar results obtained. h, Representative image of the interpeduncular nucleus (IPN) and ventral tegmental area (VTA) in mice expressing eGFP in LHb neurons. Experimental images were obtained from 3 biologically independent mice, three slices per mouse, with similar results obtained. i, Representative image of the rostromedial tegmental nucleus (RMTg) in mice expressing eGFP in LHb neurons. Experimental images were obtained from 3 biologically independent mice, three slices per mouse, with similar results obtained. j, Representative image of the RMTg and anterior dorsal and median raphe nuclei (DRN and MRN) in mice expressing eGFP in LHb neurons. Experimental images were obtained from 3 biologically independent mice, three slices per mouse, with similar results obtained. Scale bars= 500 μm.