Table 1.
Developmental efficiency of SCNT embryos derived from donor cells with Sfmbt2 miRNA KO or placenta-specific imprinted genes KO.
| Genotype of donor cells | KO allele | N** | No. of cultured embryos | No. of cleaved embryos (%) | No. of embryos transferred (ET) | No. of recipient females | No. of implantations (%/ET) | No. of live pups born (%/ET) | No. of placentas without pups (%/ET) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wild type | – | 3 | 167 | 152 | (91.0) | 101 | 4 | 48 | (47.5) | 3 | (3.0) | 4 | (4.0) |
| Sfmbt2 KO | m or p | 6 | 382 | 364 | (94.3) | 234 | 12 | 97 | (41.5) | 6 | (2.6) | 4 | (1.7) |
| Gab1 KO | m | 2 | 277 | 225 | (81.2) | 203 | 9 | 75 | (36.9) | 3 | (1.5) | 7 | (3.4) |
| Sfmbt2 miRNA KO | m | 2 | 102 | 88 | (86.3) | 75 | 3 | 27 | (36.0) | 5 | (6.7) | 2 | (2.7) |
| Sfmbt2 miRNA/Gab1 KO | m/m | 2 | 187 | 164 | (87.7) | 134 | 5 | 60 | (44.8) | 9 | (6.7) | 3 | (2.2) |
| Slc38a4 KO* | m | 2 | 84 | 79 | (94.0) | 40 | 2 | 21 | (52.5) | 3 | (7.5) | 1 | (2.5) |
| Wild type* | – | 3 | 201 | 194 | (96.5) | 179 | 9 | 110 | (61.5) | 15 | (8.4) | 3 | (1.7) |
*Embryos injected with Kdm4d mRNA instead of treatment with trichostatin A/latrunculin A. Data for the wild type are those reported by Matoba et al.16.
**Number of experimental replicates.