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. 2020 May 1;11:2138. doi: 10.1038/s41467-020-16028-8

Fig. 7. ET-1 signaling is reactivated in the adult mouse SVZ following demyelination.

Fig. 7

a Expression of ET-1 and Ednrb in the adult SVZ of WT mice 7 days following injection of saline or lysolecithin (LPC) into the SCWM. Images are representative of three WT mice. Scale bar = 25 μm. b Experimental strategy. c Coronal sections of adult WT and ET-1 cKO SVZ 7 days following LPC injection. Scale bar = 25 μm. d Quantification of the percentage of proliferating NSCs in the SVZ (Saline: n = 5 WT, 5 ET-1 cKO mice. LPC: n = 5 WT, 3 ET-1 cKO mice). *p value = 0.0284 (WT saline versus WT LPC); *p value = 0.0156 (WT LPC versus ET-1 cKO LPC) (two-way ANOVA with Tukey’s multiple comparisons test). e Quantification of the percentage of proliferating OPCs in the SVZ (Saline: n = 5 WT, 4 ET-1 cKO mice. LPC: n = 4 WT, 3 ET-1 cKO mice). **p value = 0.0097; *p value = 0.0364 (two-way ANOVA with Tukey’s multiple comparisons test). f Quantification of Sp8+ Dcx+ NPCs in the SVZ (Saline: n = 5 WT, 4 ET-1 cKO mice. LPC: n = 4 WT, 4 ET-1 cKO mice). g RNAscope labeling of coronal sections of adult WT and ET-1 cKO SVZ 7 days following LPC injection. Arrows point to red puncta within Olig2+ cells. Scale bar = 10 μm. h Quantification of the percentage of Jag1+ cells in the dorsal SVZ (n = 3 WT, 4 ET-1 cKO mice). **p value = 0.0086 (two-way ANOVA with Tukey’s multiple comparisons test). i Quantification of the percentage of Hes5+ cells in the dorsal SVZ (n = 3 WT, 4 ET-1 cKO mice). ***p value = 0.0009 (two-way ANOVA with Tukey’s multiple comparisons test). j Quantification of the percentage of Gsx1+ OPCs in the dorsal SVZ (n = 3 mice both groups). ***p value = 0.0001 (WT saline versus WT LPC); ***p value = 0.0003 (WT LPC versus ET-1 cKO LPC) (two-way ANOVA with Tukey’s multiple comparisons test). k Quantification of the percentage of S100b+ OPCs in the dorsal SVZ (n = 3 WT, 4 ET-1 cKO mice). *p value = 0.0187 (two-way ANOVA with Tukey’s multiple comparisons test). LV lateral ventricle. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.