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. 2020 May 1;11:2138. doi: 10.1038/s41467-020-16028-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a, b ET-1 staining in the adult human SVZ of CARASAL patients and age-matched controls. Scale bars = 50 μm. c Quantification of the number of ET-1+ pixels in the SVZ (n = 20 images of the SVZ of two control and two CARASAL patients). d, e ET-1+ GFAP+ astrocytes in the SVZ of CARASAL patients, compared with controls. Scale bars = 25 μm. f Quantification of the number of GFAP+ ET-1+ cells per area in the SVZ (n = 37 images of the SVZ of two control patients, 39 images of the SVZ of two CARASAL patients). ***p value < 0.0001 (Welch’s t-test). g, h PDGFRα staining in the adult human SVZ of CARASAL patients and age-matched controls. Scale bars = 50 μm. i Quantification of the number of PDGFRα+ cells per area in the SVZ (n = 10 images of the SVZ of two control patients, 15 images of the SVZ of two CARASAL patients). **p value = 0.0044 (Welch’s t-test). Data are presented as mean values ± SEM. Source data are provided as a Source Data file.